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rabbit polyclonal rag1 k-20 antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology rabbit polyclonal rag1 k-20 antibody
    A. Changes in reduced (GSH) and oxidized (GSSG) glutathione levels show increased antioxidant capacity in blood of mice after chronic adaptation to the 10% oxygen condition. Data shown as mean ± SEM, with n = 4. B. Decreased ROS levels measured by DCF FACS in cells isolated from the thymus of mice in 10% oxygen. Data shown as mean ± SEM, with n = 5. C. Representative images of decreased oxidative DNA damage detected by avidin-FITC (Av-FITC) staining for 8-oxoG in thymus tissue from p53−/− mouse exposed to 10% versus 21% oxygen for 2 to 4 wk. Nuclei counterstaining with DAPI show similar densities in the tissue. Scale bar, 100 µm (originally 63× magnification). D. Decreased oxidative DNA damage quantified by 8-oxoG enzyme-linked immunosorbent assay (ELISA) in thymus tissue from p53−/− mice exposed to 10% compared to 21% oxygen. Absolute value of 8-oxoG (pg/µg genomic DNA) shown as mean ± SEM, with n = 3. E. Increased relative telomere length measured by RT-PCR of genomic DNA from thymus tissue of p53−/− mice exposed to 10% versus 21% oxygen. Data shown as mean ± SEM, with n = 3. F. Decreased <t>RAG1</t> protein level measured by western blotting in 10% versus 21% oxygen. Samples shown are from two separate animals in each oxygen condition and β-actin serves as protein loading control.
    Rabbit Polyclonal Rag1 K 20 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+rag1+k-20+antibody/anti+rag1/pmc03093396-21-8-12
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal rag1 k-20 antibody - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Ambient Oxygen Promotes Tumorigenesis"

    Article Title: Ambient Oxygen Promotes Tumorigenesis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0019785

    A. Changes in reduced (GSH) and oxidized (GSSG) glutathione levels show increased antioxidant capacity in blood of mice after chronic adaptation to the 10% oxygen condition. Data shown as mean ± SEM, with n = 4. B. Decreased ROS levels measured by DCF FACS in cells isolated from the thymus of mice in 10% oxygen. Data shown as mean ± SEM, with n = 5. C. Representative images of decreased oxidative DNA damage detected by avidin-FITC (Av-FITC) staining for 8-oxoG in thymus tissue from p53−/− mouse exposed to 10% versus 21% oxygen for 2 to 4 wk. Nuclei counterstaining with DAPI show similar densities in the tissue. Scale bar, 100 µm (originally 63× magnification). D. Decreased oxidative DNA damage quantified by 8-oxoG enzyme-linked immunosorbent assay (ELISA) in thymus tissue from p53−/− mice exposed to 10% compared to 21% oxygen. Absolute value of 8-oxoG (pg/µg genomic DNA) shown as mean ± SEM, with n = 3. E. Increased relative telomere length measured by RT-PCR of genomic DNA from thymus tissue of p53−/− mice exposed to 10% versus 21% oxygen. Data shown as mean ± SEM, with n = 3. F. Decreased RAG1 protein level measured by western blotting in 10% versus 21% oxygen. Samples shown are from two separate animals in each oxygen condition and β-actin serves as protein loading control.
    Figure Legend Snippet: A. Changes in reduced (GSH) and oxidized (GSSG) glutathione levels show increased antioxidant capacity in blood of mice after chronic adaptation to the 10% oxygen condition. Data shown as mean ± SEM, with n = 4. B. Decreased ROS levels measured by DCF FACS in cells isolated from the thymus of mice in 10% oxygen. Data shown as mean ± SEM, with n = 5. C. Representative images of decreased oxidative DNA damage detected by avidin-FITC (Av-FITC) staining for 8-oxoG in thymus tissue from p53−/− mouse exposed to 10% versus 21% oxygen for 2 to 4 wk. Nuclei counterstaining with DAPI show similar densities in the tissue. Scale bar, 100 µm (originally 63× magnification). D. Decreased oxidative DNA damage quantified by 8-oxoG enzyme-linked immunosorbent assay (ELISA) in thymus tissue from p53−/− mice exposed to 10% compared to 21% oxygen. Absolute value of 8-oxoG (pg/µg genomic DNA) shown as mean ± SEM, with n = 3. E. Increased relative telomere length measured by RT-PCR of genomic DNA from thymus tissue of p53−/− mice exposed to 10% versus 21% oxygen. Data shown as mean ± SEM, with n = 3. F. Decreased RAG1 protein level measured by western blotting in 10% versus 21% oxygen. Samples shown are from two separate animals in each oxygen condition and β-actin serves as protein loading control.

    Techniques Used: Isolation, Avidin-Biotin Assay, Staining, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

    Related Articles

    Isolation:

    Article Title: Ambient Oxygen Promotes Tumorigenesis
    Article Snippet: Antibodies used in the study are as follows: Rabbit polyclonal RAG1 (K-20, Santa Cruz Biotechnology); monoclonal phospho-S-139 histone H2AX (γ-H2AX, clone JBW301, Millipore); monoclonal β-actin (AC-15, Sigma); rabbit monoclonal β-catenin (6B3) and cyclin D1 (DCS6, Cell Signaling). .. C

    Avidin-Biotin Assay:

    Article Title: Ambient Oxygen Promotes Tumorigenesis
    Article Snippet: Antibodies used in the study are as follows: Rabbit polyclonal RAG1 (K-20, Santa Cruz Biotechnology); monoclonal phospho-S-139 histone H2AX (γ-H2AX, clone JBW301, Millipore); monoclonal β-actin (AC-15, Sigma); rabbit monoclonal β-catenin (6B3) and cyclin D1 (DCS6, Cell Signaling). .. C

    Staining:

    Article Title: Ambient Oxygen Promotes Tumorigenesis
    Article Snippet: Antibodies used in the study are as follows: Rabbit polyclonal RAG1 (K-20, Santa Cruz Biotechnology); monoclonal phospho-S-139 histone H2AX (γ-H2AX, clone JBW301, Millipore); monoclonal β-actin (AC-15, Sigma); rabbit monoclonal β-catenin (6B3) and cyclin D1 (DCS6, Cell Signaling). .. C

    Enzyme-linked Immunosorbent Assay:

    Article Title: Ambient Oxygen Promotes Tumorigenesis
    Article Snippet: Antibodies used in the study are as follows: Rabbit polyclonal RAG1 (K-20, Santa Cruz Biotechnology); monoclonal phospho-S-139 histone H2AX (γ-H2AX, clone JBW301, Millipore); monoclonal β-actin (AC-15, Sigma); rabbit monoclonal β-catenin (6B3) and cyclin D1 (DCS6, Cell Signaling). .. C

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Ambient Oxygen Promotes Tumorigenesis
    Article Snippet: Antibodies used in the study are as follows: Rabbit polyclonal RAG1 (K-20, Santa Cruz Biotechnology); monoclonal phospho-S-139 histone H2AX (γ-H2AX, clone JBW301, Millipore); monoclonal β-actin (AC-15, Sigma); rabbit monoclonal β-catenin (6B3) and cyclin D1 (DCS6, Cell Signaling). .. C

    Western Blot:

    Article Title: Ambient Oxygen Promotes Tumorigenesis
    Article Snippet: Antibodies used in the study are as follows: Rabbit polyclonal RAG1 (K-20, Santa Cruz Biotechnology); monoclonal phospho-S-139 histone H2AX (γ-H2AX, clone JBW301, Millipore); monoclonal β-actin (AC-15, Sigma); rabbit monoclonal β-catenin (6B3) and cyclin D1 (DCS6, Cell Signaling). .. C

    Control:

    Article Title: Ambient Oxygen Promotes Tumorigenesis
    Article Snippet: Antibodies used in the study are as follows: Rabbit polyclonal RAG1 (K-20, Santa Cruz Biotechnology); monoclonal phospho-S-139 histone H2AX (γ-H2AX, clone JBW301, Millipore); monoclonal β-actin (AC-15, Sigma); rabbit monoclonal β-catenin (6B3) and cyclin D1 (DCS6, Cell Signaling). .. C



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    Santa Cruz Biotechnology rabbit polyclonal rag1 k-20 antibody
    A. Changes in reduced (GSH) and oxidized (GSSG) glutathione levels show increased antioxidant capacity in blood of mice after chronic adaptation to the 10% oxygen condition. Data shown as mean ± SEM, with n = 4. B. Decreased ROS levels measured by DCF FACS in cells isolated from the thymus of mice in 10% oxygen. Data shown as mean ± SEM, with n = 5. C. Representative images of decreased oxidative DNA damage detected by avidin-FITC (Av-FITC) staining for 8-oxoG in thymus tissue from p53−/− mouse exposed to 10% versus 21% oxygen for 2 to 4 wk. Nuclei counterstaining with DAPI show similar densities in the tissue. Scale bar, 100 µm (originally 63× magnification). D. Decreased oxidative DNA damage quantified by 8-oxoG enzyme-linked immunosorbent assay (ELISA) in thymus tissue from p53−/− mice exposed to 10% compared to 21% oxygen. Absolute value of 8-oxoG (pg/µg genomic DNA) shown as mean ± SEM, with n = 3. E. Increased relative telomere length measured by RT-PCR of genomic DNA from thymus tissue of p53−/− mice exposed to 10% versus 21% oxygen. Data shown as mean ± SEM, with n = 3. F. Decreased <t>RAG1</t> protein level measured by western blotting in 10% versus 21% oxygen. Samples shown are from two separate animals in each oxygen condition and β-actin serves as protein loading control.
    Rabbit Polyclonal Rag1 K 20 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+rag1+k-20+antibody/anti+rag1/pmc03093396-21-8-12
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal rag1 k-20 antibody - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

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    A. Changes in reduced (GSH) and oxidized (GSSG) glutathione levels show increased antioxidant capacity in blood of mice after chronic adaptation to the 10% oxygen condition. Data shown as mean ± SEM, with n = 4. B. Decreased ROS levels measured by DCF FACS in cells isolated from the thymus of mice in 10% oxygen. Data shown as mean ± SEM, with n = 5. C. Representative images of decreased oxidative DNA damage detected by avidin-FITC (Av-FITC) staining for 8-oxoG in thymus tissue from p53−/− mouse exposed to 10% versus 21% oxygen for 2 to 4 wk. Nuclei counterstaining with DAPI show similar densities in the tissue. Scale bar, 100 µm (originally 63× magnification). D. Decreased oxidative DNA damage quantified by 8-oxoG enzyme-linked immunosorbent assay (ELISA) in thymus tissue from p53−/− mice exposed to 10% compared to 21% oxygen. Absolute value of 8-oxoG (pg/µg genomic DNA) shown as mean ± SEM, with n = 3. E. Increased relative telomere length measured by RT-PCR of genomic DNA from thymus tissue of p53−/− mice exposed to 10% versus 21% oxygen. Data shown as mean ± SEM, with n = 3. F. Decreased RAG1 protein level measured by western blotting in 10% versus 21% oxygen. Samples shown are from two separate animals in each oxygen condition and β-actin serves as protein loading control.

    Journal: PLoS ONE

    Article Title: Ambient Oxygen Promotes Tumorigenesis

    doi: 10.1371/journal.pone.0019785

    Figure Lengend Snippet: A. Changes in reduced (GSH) and oxidized (GSSG) glutathione levels show increased antioxidant capacity in blood of mice after chronic adaptation to the 10% oxygen condition. Data shown as mean ± SEM, with n = 4. B. Decreased ROS levels measured by DCF FACS in cells isolated from the thymus of mice in 10% oxygen. Data shown as mean ± SEM, with n = 5. C. Representative images of decreased oxidative DNA damage detected by avidin-FITC (Av-FITC) staining for 8-oxoG in thymus tissue from p53−/− mouse exposed to 10% versus 21% oxygen for 2 to 4 wk. Nuclei counterstaining with DAPI show similar densities in the tissue. Scale bar, 100 µm (originally 63× magnification). D. Decreased oxidative DNA damage quantified by 8-oxoG enzyme-linked immunosorbent assay (ELISA) in thymus tissue from p53−/− mice exposed to 10% compared to 21% oxygen. Absolute value of 8-oxoG (pg/µg genomic DNA) shown as mean ± SEM, with n = 3. E. Increased relative telomere length measured by RT-PCR of genomic DNA from thymus tissue of p53−/− mice exposed to 10% versus 21% oxygen. Data shown as mean ± SEM, with n = 3. F. Decreased RAG1 protein level measured by western blotting in 10% versus 21% oxygen. Samples shown are from two separate animals in each oxygen condition and β-actin serves as protein loading control.

    Article Snippet: Antibodies used in the study are as follows: Rabbit polyclonal RAG1 (K-20, Santa Cruz Biotechnology); monoclonal phospho-S-139 histone H2AX (γ-H2AX, clone JBW301, Millipore); monoclonal β-actin (AC-15, Sigma); rabbit monoclonal β-catenin (6B3) and cyclin D1 (DCS6, Cell Signaling).

    Techniques: Isolation, Avidin-Biotin Assay, Staining, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control