rabbit polyclonal rag1 k-20 antibody (Santa Cruz Biotechnology)
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Rabbit Polyclonal Rag1 K 20 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+rag1+k-20+antibody/anti+rag1/pmc03093396-21-8-12
Average 90 stars, based on 1 article reviews
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1) Product Images from "Ambient Oxygen Promotes Tumorigenesis"
Article Title: Ambient Oxygen Promotes Tumorigenesis
Journal: PLoS ONE
doi: 10.1371/journal.pone.0019785
Figure Legend Snippet: A. Changes in reduced (GSH) and oxidized (GSSG) glutathione levels show increased antioxidant capacity in blood of mice after chronic adaptation to the 10% oxygen condition. Data shown as mean ± SEM, with n = 4. B. Decreased ROS levels measured by DCF FACS in cells isolated from the thymus of mice in 10% oxygen. Data shown as mean ± SEM, with n = 5. C. Representative images of decreased oxidative DNA damage detected by avidin-FITC (Av-FITC) staining for 8-oxoG in thymus tissue from p53−/− mouse exposed to 10% versus 21% oxygen for 2 to 4 wk. Nuclei counterstaining with DAPI show similar densities in the tissue. Scale bar, 100 µm (originally 63× magnification). D. Decreased oxidative DNA damage quantified by 8-oxoG enzyme-linked immunosorbent assay (ELISA) in thymus tissue from p53−/− mice exposed to 10% compared to 21% oxygen. Absolute value of 8-oxoG (pg/µg genomic DNA) shown as mean ± SEM, with n = 3. E. Increased relative telomere length measured by RT-PCR of genomic DNA from thymus tissue of p53−/− mice exposed to 10% versus 21% oxygen. Data shown as mean ± SEM, with n = 3. F. Decreased RAG1 protein level measured by western blotting in 10% versus 21% oxygen. Samples shown are from two separate animals in each oxygen condition and β-actin serves as protein loading control.
Techniques Used: Isolation, Avidin-Biotin Assay, Staining, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control
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Isolation:Article Title: Ambient Oxygen Promotes Tumorigenesis Article Snippet: Antibodies used in the study are as follows: Avidin-Biotin Assay:Article Title: Ambient Oxygen Promotes Tumorigenesis Article Snippet: Antibodies used in the study are as follows: Staining:Article Title: Ambient Oxygen Promotes Tumorigenesis Article Snippet: Antibodies used in the study are as follows: Enzyme-linked Immunosorbent Assay:Article Title: Ambient Oxygen Promotes Tumorigenesis Article Snippet: Antibodies used in the study are as follows: Reverse Transcription Polymerase Chain Reaction:Article Title: Ambient Oxygen Promotes Tumorigenesis Article Snippet: Antibodies used in the study are as follows: Western Blot:Article Title: Ambient Oxygen Promotes Tumorigenesis Article Snippet: Antibodies used in the study are as follows: Control:Article Title: Ambient Oxygen Promotes Tumorigenesis Article Snippet: Antibodies used in the study are as follows: |